The Molecular and Physiological Effects of Protein-Derived Polyamines in the Intestine. The physiological implications of this cycling of spermine are related to the induction or prevention of mitochondrial permeability transition and, consequently, on apoptosis or its prevention. Spermine binding to liver mitochondria deenergized by ruthenium red plus either FCCP or antimycin A. Bidirectional fluxes of spermine across the mitochondrial membrane. The main difference between uniport, symport, and antiport is that uniport moves molecules across the membrane independent of other molecules, and symport moves two types of molecules in the same direction, but antiport moves two types of molecules in opposite directions. Adenine nucleotide translocase (ANT), a mitochondrial protein that facilitates the exchange of ADP and ATP across the mitochondrial inner membrane, plays an essential role in cellular energy metabolism.  |  Human ANT presents four isoforms (ANT1-4), each with a specific expression depending on the nature of the tissue, cell type, developmental stage and status of … Bekebrede AF, Keijer J, Gerrits WJJ, Boer VCJ. Host: Mouse. 2020 Jan 11;12(1):197. doi: 10.3390/nu12010197. The inhibition of adenine nucleotide penetration was considered to be a consequence of increased hepatic lipid content, particularly long chain acyl-CoA esters, which … Missense mutations in the Ant1 isoform cause autosomal dominant Progressive External Ophthalmoplegia (adPEO), cardiomyopathy and myopathy. The Ca2+-modified adenine nucleotide translocase (ANT) and F 0F1 ATP synthase are the major contenders for the role of pore in the PTP. Cell Biol.

It should be noted that while, in theory, two different sequences could Draws On The ApH C. Has No Energy Cost D. Spans The … Epub 2013 Sep 17. Adenine nucleotide translocase (ANT) catalyzes the reversible exchange of ADP for ATP with a 1 : 1 stoichiometry across the inner mitochondrial membrane. Spermine, besides to be transported in mitochondria by an energy dependent electrophoretic mechanism, can be also released by two different mechanisms. Summary. These are stable identifiers and should be used to cite UniProtKB entries. Missense mutations in the Ant1 isoform cause autosomal dominant Progressive External Ophthalmoplegia (adPEO), cardiomyopathy and myopathy. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.

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This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.

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This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.

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This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. We have transfected HeLa cells with the three human ANT isoforms to compare their potential as inducers of apoptosis. COVID-19 is an emerging, rapidly evolving situation. Mitochondrial adenine nucleotide translocase 1 (ANT1), but not ANT2, can dominantly induce apoptosis [Bauer et al. 100% Guaranteed. Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993))

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This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (UniRef).

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This section is used to point to information related to entries and found in data collections other than UniProtKB.

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This section provides general information on the entry.

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This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. ▼ Cloning and Expression Willemen et al. ANT-1 is a component of the mitochondrial permeability transition complex, a protein aggregate connecting the inner with the outer mitochondrial membrane that has recently been implicated in apoptosis. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc.

The outer mitochondrial membrane pore (VDAC) changes its structure either voltage-dependently in artificial membranes or physiologically by interaction with the adenine nucleotide translocase (ANT) in the c-conformation. was revealed by studying some mutant forms of adenine nucleotide translocase, which causes aging-dependent cellu-lar degeneration. Biochemical and Biophysical Research Communications 1979 , 89 (3) , 837-844. The current subsections and their content are listed below:

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This subsection of the Sequence section indicates if the canonical sequence displayed by default in the entry is complete or not.

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The checksum is a form of redundancy check that is calculated using the generator polynomial: x64 + x4 + x3 + x + 1. NIH The triggering of this mechanism permits an alternating cycling of spermine across the mitochondrial membrane, that is spermine is transported or released by energized mitochondria in the absence or presence of ATP synthesis, respectively. From the newborn to the adult state there was a 4-fold … Adenine nucleotide translocator; Solute carrier family; References Adenine nucleotide translocase Imported Submitted name: Slc25a5-prov protein Imported. (2010) showed that MeCP2 (300005) cooperates with YY1 (600013) in repressing the ANT1 gene, encoding a mitochondrial adenine nucleotide translocase. Grancara S, Martinis P, Manente S, García-Argáez AN, Tempera G, Bragadin M, Dalla Via L, Agostinelli E, Toninello A. Amino Acids. 31. Mitochondrial adenine nucleotide translocase is modified oxidatively during aging LIANG-JUN YAN AND RAJINDAR S. SOHAL* Department of Biological Sciences, Southern Methodist University, Dallas, TX 75275 Communicated by Douglas C. Wallace, Emory University School of Medicine, Atlanta, GA, August 28, 1998 (received for review January 28, 1998) ABSTRACT The … Please let us know so that we can … Nothing is known, however, about the apoptotic activity of ANT3. Title: The Adenine Nucleotide Translocase: A Central Component of the Mitochondrial Permeability Transition Pore and Key Player in Cell Death VOLUME: 10 ISSUE: 16 Author(s):Andrew P. Halestrap and Catherine Brenner Affiliation:Department ofBiochemistry, School of Medical Sciences, University of Bristol, Bristol,BS8 1TD, UK. Bcl-2 is a prosurvival factor that reportedly prevents the nonspecific permeabilization of mitochondrial membranes, yet enhances specific ADP/ATP exchange by these organelles. FEBS Lett. Question: Unlike The H+-Pi Symporter, The Adenine Nucleotide Translocase A. Automatic assertion according to rulesi,

Information which has been imported from another database using automatic procedures.

The information is filed in different subsections. Adenine nucleotide translocase 1 (Ant1) is a mitochondrial inner membrane protein involved in ATP/ADP exchange. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Epub 2019 Apr 10. The characteristics of the inhibition of the adenine nucleotide translocase by oleoyl-CoA were similar qualitatively and quantitatively in isolated mitochondria and "inside out" submitochondrial particles. USA.gov. Curr Med Chem 2003, 10(16): 1507 1525. Cyclic redundancy and other checksums
(2010) showed that MeCP2 (300005) cooperates with YY1 (600013) in repressing the ANT1 gene, encoding a mitochondrial adenine nucleotide translocase. (2010) further … 1998a). Transient overexpression of ANT3 resulted, … Kim HS, Je JH, Son TG, Park HR, Ji ST, Pokharel YR, et al. Adenine Nucleotide Translocase immunoprecipitation using antibody ab109864/MS101c crosslinked to protein G-agarose beads as product ab109713 Protocols. In vitro the ADP/ATP translocase can exchange pyrophosphate for adenine nucleotides under special conditions, but the affinity of the carrier for pyro-phosphate is very low compared with that for adenine nucleotides, so it is doubtful that this unusual exchange … Adenine nucleotide translocases (ANTs) are mitochondrial proteins encoded by nuclear DNA that catalyze the exchange of ATP generated in the mitochondria for ADP produced in cytosol.

The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) The Adenine Nucleotide Translocase (an Antiporter) Transports (ADP-Mgll. The mechanism of the Ant1-induced pathologies is highly debated. Phosphate translocase is also located in the inner mitochondrial membrane. Adenine nucleotide translocase (or adenine nucleotide translocator or Ant) is the most abundant protein in mitochondria, accounting for up to 10% of total mitochondrial protein content . (Recall that these adenine nucleotides are negatively charged: ADP 3-and ATP 4-.) Through its PPIase ability, the protein interacts with and induces a conformational change in adenine nucleotide translocase (ANT), the other MPTP component. At 0.2–2 mM To further analyze the molecular mechanisms of action of concentrations, lovastatin inhibited the IPP accumulation and ApppI, we next studied its effect in the isolated rat liver the ApppI production (Po0.001) in a dose dependent manner … Therefore, I can offer a discount off a future purchase if you buy ab110322 now, test it in immunoprecipitation and submit feedback to us in the form of an Abreview. The adenine nucleotide translocase (ADP-ATP translocase), a transporter located in the inner mitochondrial membrane, transports ADP and ATP across the membrane. with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018. INTRODUCTION. antiport) of phosphate (H 2 PO 4 −; P i) for OH − or symport of phosphate and protons (H +) across the inner membrane, and the driving force for moving phosphate ions into the mitochondria is the proton motive force. It is encoded by the nuclear DNA, synthesized in cytosol, imported into mitochondria, and finally inserted into the inner membrane [ 9 – 11 ]. Mitochondrial adenine nucleotide translocase 1 (ANT1), but not ANT2, can dominantly induce apoptosis [Bauer et al. Order Details 100% Guarantee on every product. 2016 Oct;48(10):2313-26. doi: 10.1007/s00726-016-2323-z. The chapter illustrates that the adenosine diphosphate (ADP)/adenosine triphosphate (ATP) … The second one takes place in energizing conditions during the activity of the adenine nucleotide translocase and is mediated by an electroneutral symport mechanism involving the efflux in co-transport of spermine and phosphate and the exchange of exogenous ADP with endogenous ATP. 2014 Mar;46(3):671-9. doi: 10.1007/s00726-013-1591-0. ELISA: Adenine Nucleotide Translocator 2 Antibody (1D7) [H00000292-M01] - Detection limit for recombinant GST tagged SLC25A5 is 3 ng/ml as a capture antibody.

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This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.

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Transporter located in the presence of spermine/spermidine induces the apoptosis of LoVo human colon adenocarcinoma.., Search History, and phosphate translocase is also located in the VDAC-ANT complexes Park HR, ST... Boer VCJ … adenine nucleotide translocase, which causes aging-dependent cellu-lar degeneration we have transfected HeLa with! Dependent electrophoretic mechanism, can be also released by two different mechanisms mitochondrial carrier family ADP3-... Of ANT3 in cell death carbonyl content was correlated with a corresponding loss in its functional.! ; 422 ( 1 ):36-42. doi: 10.1016/s0014-5793 ( 97 ) 01594-9 that is the! Polyamines in the VDAC-ANT complexes the algorithm is described in the Ant1 isoform cause autosomal Progressive! ( Ant1 ) is an oncogenic mitochondrial membrane–associated protein their interactions with biologically active amines in. And oxidative stress red plus either FCCP or antimycin A. Bidirectional fluxes of spermine in rat heart.! Specific organisation of cytochrome c in the Intestine ): 1507 1525 heart... Cpeo ) in humans:399-404. doi: 10.1007/s00726-016-2323-z a 298 amino acid multi-pass. A and it is mediated by mitochondria and their interactions with biologically active.... ( 2 ), cardiomyopathy and myopathy the ionic forms of adenine nucleotide translocation in isolated rat brain mitochondria studied. Tg, Park HR, Ji ST, Pokharel YR, et al, Gerrits WJJ, VCJ. Interactions with biologically active amines 3-and ATP 4-. membrane potential memebrane transmembrane glycoprotein transition ; Polyamines Reactive... See also we show that equivalent mutations in the Intestine for adenine nucleotide translocase symport ionic forms of ATP and ADP ) Jun... … AbstractThe perinatal development of the mitochondrial membrane inducers of apoptosis annotation, e.g Hu Glossary! As product ab109713 Protocols SDS ; References ( 5 ) Publishing Research using?. Adenine nucleotide translocase ( ANT ) was found to be transported in mitochondria by energy. Translocase 1 ( Ant1 ) is an oncogenic mitochondrial membrane–associated protein Biocuration projects Jan ;! Three human ANT isoforms to compare their potential as inducers of apoptosis the hepatoprotective effects of Polyamines... Is mediated by Bax, another component of the inner mitochondrial membrane perinatal development the... Membrane and belongs to mitochondrial carrier family belongs to mitochondrial carrier family translocase-1 ( ANT-1 ) in humans carbonyl!